![]() ![]() Image Rescaling Options: If the image is larger that 1300 pixels width, it will be rescaled to speed up the processing.Native Spatial Scale: Input the current image scale in pixels/microns.Image Native Resolution: Displays the imported image resolution.Microscope Magnification: Displays the working 20x objective magnification.Settings Dialog: Input the spatial scale obtained from the image metadata or microscope.Īfter importing a TIFF image and executing the counter, the following settings should be selected: These measurements can be performed in Fiji with the freehand selection tool, after setting the spatial scale factor. Minimum Cell Size & Maximum Cell Area (µm 2): These values should be determined upfront, according to your image dataset.Microscope Spatial Scale: Pixels/microns.Digital Image: Files should be in TIFF format.TUNEL Cell Counter: Macro should be installed in Fiji (ImageJ).The following are required to execute this tool: TUNEL Cell Counter: Input a fluorescent-labeled retinal image for ONL & INL layer segmentation and cell quantitation. It segments retinal outer nuclear (ONL) and inner nuclear layers (INL) and quantitates fluorescent-labelled cells in these layers. TUNEL Cell Counter is a customizable tool that processes digital images from retinal cryosections. The current components of these toolkit are: TUNEL Cell Counter and RETINA Cell Heatmap. ![]() The purpose of the RETINA Analysis Toolkit is to perform fast quantitation of digital RGB images from retina cryosections, acquired by fluorescent microscopes. RETINA Analysis Toolkit is a free macro toolkit designed and developed for Fiji (ImageJ).
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